Fig. 1.

EpoL induced a protective effect against stress induced by chronic Aβ treatment. (A) Viability assay in PC12 cells treated with oligomers of Aβ₄₀ (1uM) for 24 h and Epo or EpoL at different concentrations. The percentage of viability response was evaluated by MTT. (B) Viability assay using a specific antibody to block interaction between EpoR and EpoL or EpoL. (C) Evaluation of the relative expression of the Bcl-2 gene using β-actin as a housekeeping gene after 24 h of treatment with Aβ and co-treatment with Epo or EpoL, at the neuroprotective concentrations observed. Values are mean ± SEM, n = 3 using one-way ANOVA and Dunns test. *:p < 0.05; **:p < 0.01 versus PC12 cells treated with Ab; ++++:p < 0.0001 versus control cells.