Fig. 3.
The intracellular calcium modulating effects of EpoL in Aβ treated neurons is EpoR dependent. (A) Hippocampal neurons were treated for 24 h with oligomers of Aβ40 and co-incubated with EpoL and a specific antibody at neuroprotective concentrations, as represented in the schematic. (B) Quantification of percentage frequency of calcium transients in Fluo4AM loaded cells treated for 24 h with Aβ or Aβ+EpoL or Aβ+ EpoL + Aβ, respectively. (C) Representative images of calcium transients during 200 s, under the indicated treatments. Values are mean ± SEM, n = 3 using one-way ANOVA and Dunns test. ****:p < 0.0001 versus neuronal culture treated with Aβ; ++++:p < 0.0001 versus control cells; ####:p < 0.0001 versus EpoL or Epo treatment.