Fig. 7.
The neuroprotective effect of EpoL in hippocampal organotypic cultures against Aβ stress depended on EpoR interaction: (A) Quantification of fluorescence signal intensity of DCF-DA/Hoechst on the CA1 region of hippocampus obtained by fluorescence images of hippocampi organotypic cultures treated for 4 days with Aβ25-35 (0.5 μM) and co-incubated with EpoL or EpoL + Anti-EpoR (Ab). (B) Quantification of PI/Hoechst signal of the CA1 region, as the same conditions used in A. Values are mean ± SEM, n = 7 using One-way ANOVA and Tukey test. ***:p < 0.001, “ns” versus Aβ; ###:p < 0.001 versus ctrl.