Fig. 8.
EpoL and Epo prevented astrogliosis induced by chronic treatment with Aβ. (A) Immunofluorescence confocal images of organotypic hippocampal cultures treated for 4 days with Aβ25-35 (0.5 μM) and co-incubated with EpoL or Epo at a neuroprotective concentration to analyze GFAP intensity signal. (B) Scheme of protocol used. (C) Mean intensity of GFPA fluorescence in the CA1 observed under each experimental condition using ImageJ software. Values are mean ± SEM, n = 3 using one-way ANOVA and Dunns test. ***:p < 0.001 versus culture treated with Aβ25-35; ++++:p < 0.001 versus control cells.