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. 2019 Apr 2;10:619. doi: 10.3389/fimmu.2019.00619

Figure 3.

Figure 3

T-helper lymphocyte subsets in co-culture with MSC. PBMCs were cultured with or without MSCs in the presence of anti-CD3/CD28 microbeads (and IL-2 for Treg analyses) for 7 days, at a MSC/PBMC ratio of 1/10. Proportions of (A) Treg (CD4+CD25+FoxP3+), (B) IL10+, (C) Th1 (IFNγ+), (D) Th2 (IL-4+), and (E) Th17 (IL-17+) cells were evaluated at day 7 by FACS. Data are presented as individual observations (or mean value if the experiment was realized in duplicates) with median. Global p-values (repeated measure ANOVA-1) are shown as well as comparisons between MSC groups and controls with Dunnett's post-hoc procedure (*p < 0.05; **p < 0.01).