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. 2019 Apr 2;10:610. doi: 10.3389/fimmu.2019.00610

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Copyright © 2019 Coronado, Solis, Hernandez and Feijóo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophils and macrophages increase in number in the gut during the inflammation process triggered in the developed feeding model. The number of neutrophils/macrophages (A), and mast cells (B) was quantified under the different conditions (m, maintenance; CTRL, control; INFL, inflammation) and time points (T0m: 18 dpf; T4: 21 dpf; T8: 25 dpf). (C) Relative mRNA levels of gzma and nkld were analyzed. Data were normalized against rpl13a and compared to T0 maintenance condition (dotted line). ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001. Experiments were done at least in three biological replicates with 20 individuals per condition.