Table 2.

Side Effect Profiles for Genome-Editing Methods

Serial No.	Parameter	ZFN	TALEN	CRISPER/Cas	Reference
1	off-target effect incidence	–	–	–	54
a	homologous recombination rate frequency	+	+	+	–
b	non-homologous end joining (NHEJ) mutation rates	+	+	++ (only with earlier versions)	55, 56
c	immune reaction susceptibility	less	less	more	57, 58
d	RNA-guided endonuclease (RGEN)-induced off-target mutatagenesis	−	−	++	59
2	cytotoxicity chances	++	+	+	–