Table 1.
Mass spectrometry parameters for analytes and internal standards. Scan speed (dwell time) and detection windows were adjusted accordingly.
| Compound | IS | Cone voltage (V) | Q1 mass (m/z) | Quantification transition | Confirmation transition | RT (min) | ||
|---|---|---|---|---|---|---|---|---|
| Q3 mass (m/z) | CE (eV) | Q3 mass (m/z) | CE (eV) | |||||
| Acetyl norfentanyl-D5 (A-D5) | - | 25 | 224.2 | 84.0 | 18 | - | - | 2.23 |
| Fentanyl-D5 (F-D5) | - | 25 | 342.2 | 105.2 | 38 | - | - | 4.76 |
| Acetylfentanyl | A-D5 | 25 | 322.2 | 188.0 | 20 | 105.0 | 36 | 4.18 |
| Acetyl norfentanyl | A-D5 | 25 | 219.2 | 84.1 | 18 | 55.2 | 36 | 2.28 |
| Alfentanil | F-D5 | 24 | 417.1 | 268.1 | 16 | 197.1 | 26 | 4.80 |
| Butyrylfentanyl | F-D5 | 30 | 351.2 | 105.0 | 40 | 188.1 | 22 | 5.11 |
| Butyrylfentanyl carboxy metabolite | F-D5 | 25 | 381.2 | 105.0 | 42 | 188.0 | 30 | 4.05 |
| Butyryl norfentanyl | A-D5 | 25 | 247.1 | 84.2 | 20 | 55.3 | 36 | 3.80 |
| Carfentanil | F-D5 | 22 | 395.2 | 113.0 | 32 | 335.0 | 16 | 5.08 |
| Cyclopropylfentanyl | F-D5 | 25 | 349.2 | 105.0 | 36 | 188.1 | 20 | 4.97 |
| Cyclopropyl norfentanyl | F-D5 | 25 | 245.2 | 84.1 | 36 | 177.1 | 20 | 3.41 |
| Despropionylfentanyl (4-ANPP) | F-D5 | 22 | 281.2 | 105.0 | 32 | 188.0 | 14 | 4.77 |
| Despropionyl para-fluorofentanyl | F-D5 | 15 | 299.1 | 105.0 | 38 | 188.1 | 16 | 4.89 |
| Fentanyl | F-D5 | 25 | 337.2 | 188.2 | 30 | 105.2 | 38 | 4.75 |
| Furanylfentanyl | F-D5 | 16 | 375.1 | 188.0 | 18 | 105.0 | 38 | 4.87 |
| Furanyl norfentanyl | A-D5 | 16 | 271.0 | 84.2 | 18 | 55.1 | 38 | 3.24 |
| Furanylethyl fentanyl | F-D5 | 25 | 327.2 | 95.1 | 35 | 178.1 | 16 | 4.18 |
| β-Hydroxyfentanyl | F-D5 | 25 | 389.2 | 111.0 | 38 | 238.0 | 16 | 5.15 |
| β-Hydroxythiofentanyl | F-D5 | 25 | 359.2 | 192.0 | 22 | 111.0 | 38 | 4.12 |
| Methoxyacetylfentanyl | A-D5 | 25 | 353.3 | 188.1 | 20 | 105.0 | 38 | 4.07 |
| Methoxyacetyl norfentanyl | A-D5 | 15 | 249.0 | 84.1 | 14 | 55.0 | 38 | 2.19 |
| Norfentanyl | F-D5 | 25 | 233.1 | 84.3 | 20 | 55.3 | 34 | 3.10 |
| Phenylacetyl fentanyl | A-D5 | 46 | 399.3 | 105.1 | 44 | 188.1 | 24 | 5.19 |
| Sufentanil | F-D5 | 16 | 387.2 | 111.0 | 38 | 238.1 | 18 | 5.15 |
| Valerylfentanyl carboxy metabolite | F-D5 | 40 | 395.3 | 105.3 | 44 | 188.1 | 26 | 4.17 |
CE, collision energy; IS, internal standard; RT, retention time.