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. 2019 Apr 2;7:184. doi: 10.3389/fchem.2019.00184

Table 4.

Validation parameters for fentanyl and analogs in hair.

Compound Determination coefficient (r2) LOD (ng/g) LOQ (ng/g) Accuracy (% error) Intra-assay precision (% CV) Inter-assay precision (% CV) Recovery (%) n = 15 Matrix effect (%) n = 15
Low n = 15 Mid n = 15 High n = 15 Low n = 15 Mid n = 15 High n = 15 Low n = 15 Mid n = 15 High n = 15
Acetylfentanyl 0.992 ± 0.008 0.004 0.012 12.2 8.6 9.7 13.6 11.0 9.7 13.9 10.2 8.6 81.2 90.6
Acetyl norfentanyl 0.994 ± 0.002 0.003 0.011 11.2 7.1 6.5 12.8 5.0 5.4 13.2 6.8 8.5 92.3 91.2
Alfentanil 0.991 ± 0.010 0.005 0.017 10.3 7.6 6.5 10.8 8.2 7.8 12.1 9.4 10.5 84.3 90.4
Butyrylfentanyl 0.995 ± 0.004 0.005 0.015 10.2 6.8 6.2 10.6 7.8 6.9 11.8 8.1 7.8 89.6 90.1
Butyrylfentanyl carboxy metabolite 0.992 ± 0.001 0.006 0.02 10.7 7.8 6.4 13.5 10.4 8.9 13.9 8.1 7.2 76.9 90.9
Butyryl norfentanyl 0.995 ± 0.001 0.006 0.018 11.2 3.5 2.8 11.8 6.1 7.8 12.5 7.9 9.1 89.7 91.1
Carfentanil 0.993 ± 0.001 0.006 0.019 10.7 5.3 4.9 12.7 8.5 6.9 12.9 9.2 8.9 88.3 90.8
Cyclopropylfentanyl 0.997 ± 0.002 0.006 0.019 12.0 9.2 10.2 10.2 7.3 8.6 7.8 9.3 11.5 93.3 91.0
Cyclopropyl norfentanyl 0.996 ± 0.005 0.006 0.018 10.2 5.2 8.8 10.4 10.0 7.9 12.8 9.4 8.7 95.7 90.3
Despropionylfentanyl (4-ANPP) 0.991 ± 0.002 0.006 0.018 12.2 5.7 5.2 13.9 11.8 10.3 14.2 10.5 8.9 79.7 90.1
Despropionyl para-fluorofentanyl 0.997 ± 0.002 0.005 0.015 13.4 9.3 5.8 13.9 3.4 5.0 14.3 5.0 6.3 88.6 91.1
Fentanyl 0.992 ± 0.004 0.003 0.013 12.5 8.2 6.7 10.2 4.7 4.3 11.1 6.1 5.8 89.1 90.7
Furanylfentanyl 0.994 ± 0.002 0.005 0.016 10.5 8.7 8.2 11.5 11.6 9.9 11.8 10.4 10.1 78.9 90.2
Furanyl norfentanyl 0.998 ± 0.004 0.003 0.012 10.7 6.5 6.0 13.6 10.0 4.5 13.0 11.2 10.8 91.2 90.1
Furanylethyl fentanyl 0.994 ± 0.005 0.003 0.014 12.9 4.9 5.7 13.6 9.5 8.4 13.9 10.5 9.5 87.9 90.4
β-Hydroxyfentanyl 0.996 ± 0.002 0.005 0.014 12.3 8.9 9.1 12.6 8.2 7.6 12.8 7.9 6.5 81.5 90.9
β-Hydroxythiofentanyl 0.994 ± 0.001 0.005 0.014 12.7 10.1 8.2 13.2 10.4 9.3 13.7 9.2 8.7 83.2 90.2
Methoxyacetylfentanyl 0.997 ± 0.002 0.005 0.016 12.0 12.3 7.4 10.4 9.7 5.4 11.9 10.0 9.1 80.3 90.5
Methoxyacetyl norfentanyl 0.997 ± 0.002 0.005 0.016 12.5 10.3 9.4 11.2 8.7 7.4 12.6 9.0 8.1 74.3 90.5
Norfentanyl 0.991 ± 0.002 0.005 0.015 12.4 8.5 8.3 12.5 10.6 9.8 12.8 10.4 10.1 88.8 90.8
Phenylacetyl fentanyl 0.992 ± 0.004 0.005 0.015 11.1 6.2 5.8 10.9 8.0 6.9 12.1 7.4 6.7 92.7 90.5
Sufentanil 0.992 ± 0.002 0.006 0.019 10.5 4.6 3.9 10.9 7.3 6.1 11.2 8.6 9.7 85.4 91.3
Valerylfentanyl carboxy metabolite 0.994 ± 0.001 0.007 0.021 11.4 10.2 9.3 10.9 8.2 7.6 11.4 7.3 6.5 94.3 90.8

Low, medium, and high quality control working solutions contained all standards at 0.01, 10, and 80 ng/g, respectively.

Analytical recovery and matrix effect are displayed as an average of low, medium, and high quality control concentrations values.

CV, coefficient of variation; LOD, limit of detection; LOQ, limit of quantification.