Table 4.
Validation parameters for fentanyl and analogs in hair.
| Compound | Determination coefficient (r2) | LOD (ng/g) | LOQ (ng/g) | Accuracy (% error) | Intra-assay precision (% CV) | Inter-assay precision (% CV) | Recovery (%) n = 15 | Matrix effect (%) n = 15 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Low n = 15 | Mid n = 15 | High n = 15 | Low n = 15 | Mid n = 15 | High n = 15 | Low n = 15 | Mid n = 15 | High n = 15 | ||||||
| Acetylfentanyl | 0.992 ± 0.008 | 0.004 | 0.012 | 12.2 | 8.6 | 9.7 | 13.6 | 11.0 | 9.7 | 13.9 | 10.2 | 8.6 | 81.2 | 90.6 |
| Acetyl norfentanyl | 0.994 ± 0.002 | 0.003 | 0.011 | 11.2 | 7.1 | 6.5 | 12.8 | 5.0 | 5.4 | 13.2 | 6.8 | 8.5 | 92.3 | 91.2 |
| Alfentanil | 0.991 ± 0.010 | 0.005 | 0.017 | 10.3 | 7.6 | 6.5 | 10.8 | 8.2 | 7.8 | 12.1 | 9.4 | 10.5 | 84.3 | 90.4 |
| Butyrylfentanyl | 0.995 ± 0.004 | 0.005 | 0.015 | 10.2 | 6.8 | 6.2 | 10.6 | 7.8 | 6.9 | 11.8 | 8.1 | 7.8 | 89.6 | 90.1 |
| Butyrylfentanyl carboxy metabolite | 0.992 ± 0.001 | 0.006 | 0.02 | 10.7 | 7.8 | 6.4 | 13.5 | 10.4 | 8.9 | 13.9 | 8.1 | 7.2 | 76.9 | 90.9 |
| Butyryl norfentanyl | 0.995 ± 0.001 | 0.006 | 0.018 | 11.2 | 3.5 | 2.8 | 11.8 | 6.1 | 7.8 | 12.5 | 7.9 | 9.1 | 89.7 | 91.1 |
| Carfentanil | 0.993 ± 0.001 | 0.006 | 0.019 | 10.7 | 5.3 | 4.9 | 12.7 | 8.5 | 6.9 | 12.9 | 9.2 | 8.9 | 88.3 | 90.8 |
| Cyclopropylfentanyl | 0.997 ± 0.002 | 0.006 | 0.019 | 12.0 | 9.2 | 10.2 | 10.2 | 7.3 | 8.6 | 7.8 | 9.3 | 11.5 | 93.3 | 91.0 |
| Cyclopropyl norfentanyl | 0.996 ± 0.005 | 0.006 | 0.018 | 10.2 | 5.2 | 8.8 | 10.4 | 10.0 | 7.9 | 12.8 | 9.4 | 8.7 | 95.7 | 90.3 |
| Despropionylfentanyl (4-ANPP) | 0.991 ± 0.002 | 0.006 | 0.018 | 12.2 | 5.7 | 5.2 | 13.9 | 11.8 | 10.3 | 14.2 | 10.5 | 8.9 | 79.7 | 90.1 |
| Despropionyl para-fluorofentanyl | 0.997 ± 0.002 | 0.005 | 0.015 | 13.4 | 9.3 | 5.8 | 13.9 | 3.4 | 5.0 | 14.3 | 5.0 | 6.3 | 88.6 | 91.1 |
| Fentanyl | 0.992 ± 0.004 | 0.003 | 0.013 | 12.5 | 8.2 | 6.7 | 10.2 | 4.7 | 4.3 | 11.1 | 6.1 | 5.8 | 89.1 | 90.7 |
| Furanylfentanyl | 0.994 ± 0.002 | 0.005 | 0.016 | 10.5 | 8.7 | 8.2 | 11.5 | 11.6 | 9.9 | 11.8 | 10.4 | 10.1 | 78.9 | 90.2 |
| Furanyl norfentanyl | 0.998 ± 0.004 | 0.003 | 0.012 | 10.7 | 6.5 | 6.0 | 13.6 | 10.0 | 4.5 | 13.0 | 11.2 | 10.8 | 91.2 | 90.1 |
| Furanylethyl fentanyl | 0.994 ± 0.005 | 0.003 | 0.014 | 12.9 | 4.9 | 5.7 | 13.6 | 9.5 | 8.4 | 13.9 | 10.5 | 9.5 | 87.9 | 90.4 |
| β-Hydroxyfentanyl | 0.996 ± 0.002 | 0.005 | 0.014 | 12.3 | 8.9 | 9.1 | 12.6 | 8.2 | 7.6 | 12.8 | 7.9 | 6.5 | 81.5 | 90.9 |
| β-Hydroxythiofentanyl | 0.994 ± 0.001 | 0.005 | 0.014 | 12.7 | 10.1 | 8.2 | 13.2 | 10.4 | 9.3 | 13.7 | 9.2 | 8.7 | 83.2 | 90.2 |
| Methoxyacetylfentanyl | 0.997 ± 0.002 | 0.005 | 0.016 | 12.0 | 12.3 | 7.4 | 10.4 | 9.7 | 5.4 | 11.9 | 10.0 | 9.1 | 80.3 | 90.5 |
| Methoxyacetyl norfentanyl | 0.997 ± 0.002 | 0.005 | 0.016 | 12.5 | 10.3 | 9.4 | 11.2 | 8.7 | 7.4 | 12.6 | 9.0 | 8.1 | 74.3 | 90.5 |
| Norfentanyl | 0.991 ± 0.002 | 0.005 | 0.015 | 12.4 | 8.5 | 8.3 | 12.5 | 10.6 | 9.8 | 12.8 | 10.4 | 10.1 | 88.8 | 90.8 |
| Phenylacetyl fentanyl | 0.992 ± 0.004 | 0.005 | 0.015 | 11.1 | 6.2 | 5.8 | 10.9 | 8.0 | 6.9 | 12.1 | 7.4 | 6.7 | 92.7 | 90.5 |
| Sufentanil | 0.992 ± 0.002 | 0.006 | 0.019 | 10.5 | 4.6 | 3.9 | 10.9 | 7.3 | 6.1 | 11.2 | 8.6 | 9.7 | 85.4 | 91.3 |
| Valerylfentanyl carboxy metabolite | 0.994 ± 0.001 | 0.007 | 0.021 | 11.4 | 10.2 | 9.3 | 10.9 | 8.2 | 7.6 | 11.4 | 7.3 | 6.5 | 94.3 | 90.8 |
Low, medium, and high quality control working solutions contained all standards at 0.01, 10, and 80 ng/g, respectively.
Analytical recovery and matrix effect are displayed as an average of low, medium, and high quality control concentrations values.
CV, coefficient of variation; LOD, limit of detection; LOQ, limit of quantification.