Figure 1.

Activation of PSCs is compromised by supernatants of PDACs with BAG3 knockdown. (A) BxPC3 or SW1990 cells were infected with control (CON) or gRNA guided BAG3 (KD) using CRISPR/Cas9 system, BAG3 expression was confirmed using Western blot. (B) IL6 mRNA expression was analyzed in the indicated cells using real-time PCR. (C) IL6 extracellular release was analyzed in the indicated cells using ELISA and standardized the control and the experimental group by cell numbers. (D–J) HPanSteC cells were incubated with conditional medium (CM) collected from BxPC3 or SW1990, αSMA, and collagen expression was analyzed using Western blot analysis (D), DNA synthesis was analyzed using Edu incorporation (E), cell proliferation was analyzed using RTCA (F,G), cell migration was analyzed using Transwell (H), and RTCA (I,J), respectively. *P < 0.01.