Table 3.
Sequences of the primers used in this work.
| Primer | Sequence (5′-3′) | GenBank reference | Tm (°C) | Product length (bp) |
|---|---|---|---|---|
| baz2a-F | GTCCCATTTCCAAACACGC | XM_017036014.1 | 58.3 | 120 |
| baz2a-R | TGGACCTTAGGGCTTTTATGAG | 58.4 | ||
| kmt2d-F | CCAGATGGAGGTGAAGACAGTC | XM_017035697.1 | 58.4 | 197 |
| kmt2d-R | GCATAAAGCACAGGCGAAAT | 58.2 | ||
| mdtet3-F | CAGCCCAATCTCAGGTATCCA | XM_017042774.1 | 59.9 | 183 |
| mdtet3-R | CTTCGCACTTCGGGTCTAAAT | 58.9 | ||
| mthfd1-F | TTCTTCCGTCTCATTTGGTCA | XM_008313708.1 | 58.0 | 158 |
| mthfd1-R | CACCTGTAGAACCAGCAGACCT | 58.5 | ||
| rps10-F | GATGCTGATGCCCAAGAAGA | XM_008320951.2 | 58.3 | 179 |
| rps10-R | CCTTGACATACCCACAGGACTT | 58.2 | ||
| sfxn2-F | TGAGAACGGGAACAAACTGG | XM_008314293.2 | 58.2 | 119 |
| sfxn2-R | GCATGATGATGGGCAGAATAA | 58.4 | ||
| slcl6a5-F | TACGCCACCGCTAACAACA | XM_008337600.2 | 58.3 | 182 |
| slcl6a5-R | CAGATACTGTTACTGAGTCCGTTGA | 58.4 | ||
| usmg5-F | AAAGGAGGGACTTCCGCTAA | XM_008317371.2 | 58.4 | 123 |
| usmg5-R | AGAGTCGTGTCCACCCATGTT | 58.9 | ||
| zfp319-F | AGCACCACTCGTCCCATAAC | XM_008311023.2 | 57.2 | 158 |
| zfp319-R | TGGAAAAGGCAGAATAGAAGAAC | 57.8 | ||
| znf574-F | TGTTTCTTCCTCTGGAGTCGC | XM_008322835.2 | 59.4 | 181 |
| znf574-R | CATGCTCTGGATGAACCCTTT | 59.1 | ||
| LNC_000230-F | AAACAACCTCCAGTAACCTTCC | novel | 57.1 | 106 |
| LNC_000230-R | TCTCTCTTGCGGGAGGACTA | 57.3 | ||
| LNC_000255-F | GTTAGAGCACGACACGACCAA | novel | 58.6 | 125 |
| LNC_000255-R | GTAAAGCCAACCAACCGACA | 58.4 | ||
| LNC_000285-F | TCAACCATCAGCGTACAGTAGG | novel | 58.0 | 153 |
| LNC_000285-R | GACGACGACGCTTTCACAAC | 58.4 | ||
| LNC_000314-F | ACCTCCGACTGTTTGTATCACC | novel | 58.7 | 155 |
| LNC_000314-R | GTTCATCCTCTGCACTGGCT | 57.3 | ||
| LNC_000360-F | GGAAATGAGTTCTGAAGTGCCT | novel | 57.8 | 190 |
| LNC_000360-R | TTCACCTGGCAGCAGTTTG | 58.5 | ||
| LNC_000562-F | GCTGCCATTCCAAGACATACA | novel | 58.5 | 114 |
| LNC_000562-R | AAAAAGCCTGAGACACCCCT | 57.9 | ||
| XR_521587.1-F | AGACTCTTGAATTGTCTGTTCATCC | XR_521587.2 | 58.7 | 158 |
| XR_521587.1-R | CAGGGTGTTTGTTTATTTGTGC | 57.7 | ||
| XR_521789.1-F | ACTTCGCCTCAGCCAATCA | XR_521789.2 | 58.7 | 95 |
| XR_521789.1-R | AACCGTGTTCTCCATCAGCA | 58.5 | ||
| XR_522182.1-F | TGGTAGCCGTTGACTTCCTT | XR_522182.2 | 57.3 | 104 |
| XR_522182.1-R | TACTTTGACCTCTGCCTCATCTT | 57.8 | ||
| XR_523541.1-F | CATGATGAGTGCTGGTGGCT | XR_523541.1 | 58.8 | 203 |
| XR_523541.1-R | TGTGCTGCTGGTTGACATAGAG | 58.8 | ||
| β-2-m-F | TTGGCTCGTGTTCGTCGTTC | XM_017034328.2 | 57.2 | 119 |
| β-2-m-R | TCAGGGTGTTGGGCTTGTTG | 58.3 |
Baz2a, bromodomain adjacent to zinc finger domain 2A; kmt2d, lysine (K)-specific methyltransferase 2D; mdtet3, methylcytosine dioxygenase TET3-like; mthfd1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1; rps10, ribosomal protein, S10 sfxn2, sideroflexin 2; slc16a5, solute carrier family 16 member 5; usmg5, up-regulated during skeletal muscle growth 5 homolog; zfp319, zinc finger protein 319-like; znf574, zinc finger protein 574; β-2-m, beta-2-microglobulin.