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The interaction between tissue factor and PAR2 in FoxO6-Tg liver. (A) PAR1, PAR2, PAR3, and PAR4 mRNA in FoxO6-Tg liver. Real-time PCR analyses were performed to determine mRNA levels in liver tissues. ∗∗p < 0.01 vs. Ntg. (B) Tissue factor (TF) mRNA level in FoxO6-Tg liver. ∗∗p < 0.01 vs. Ntg. (C) Cytosolic extracts were prepared from the liver of FoxO6-Tg mice. Immunoprecipitated TF was determined to be physically associated with PAR2 by Western blotting.
