Figure 1.

Effects of multiple superovulations on embryonic development. (A) Schedule of superovulation; (B) average number of eggs retrieved from each mouse after mating with males; (C) the incidence of oocytes with the 1st PB; (D) the cell number of blastocysts. The number of blastocysts tested was 67, 73, 64 and 74 in R0, R1, R3 and R5 groups, respectively. (E and F) Effects of multiple superovulations on embryonic quality were also examined via checking the expression of the genes, such as pluripotency-related genes Oct4, Sox2, Nanog and apoptosis-related genes Bcl2, Bax and Casp3.