Figure 6.

Tri-methylation of H3K27 level in the early embryos. Fluorescence intensity of H3K27me3 in the early embryos was examined using confocal microscopy. (A) Pronuclear embryos at the PN4 phase, n = 50 (R0), n = 50 (R1), n = 49 (R3), n = 50 (R5); (B) average fluorescence intensity of female pronucleus; (C) two-cell stage embryos, n = 53 (R0), n = 54 (R1), n = 54 (R3), n = 54 (R5); (D) average fluorescence intensity of two-cell stage embryos; (E) four-cell stage embryos, n = 44 (R0), n = 44 (R1), n = 44 (R3), n = 43 (R5); (F) average fluorescence intensity of four-cell stage embryos; (G) blastocysts, n = 46 (R0), n = 47 (R1), n = 46 (R3), n = 43; (H) average fluorescence intensity of blastocysts. Scale bars = 25 μm. Data present as mean ± s.d. Different letters indicate P < 0.05 between groups.