Interaction of MRAP2 proteins with slMCa or slMCb. Co-IP of slMCa or slMCb with four types of MRAP2 proteins: (A and B) slMRAP2, (C and D) esMRAP2-C, (E and F) zMRAP2a-C and (G and H). *IgG heavy chain. (I) Schematic diagram of four MRAP2 plasmids. N terminus and TM domain of esMRAP2-C, zMRAP2a-C and zMRAP2b-C originate from slMRAP2, and the carboxyl terminus is captured from esMRAP2, zMRAP2a or zMRAP2b.