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Representative images of HEK293 cells transiently expressing TRPV4-Flag, p.S94L-Flag, p.R269C-Flag, p.R315W-Flag, and p.T701I proteins. Immunofluorescence was performed with anti-Flag tag antibody to detect TRPV4-Flag proteins and Na-K-ATPase to label the plasma membrane. Nuclei were stained with DAPI. Scale bar = 20 μm.
