Fig. 3.

Analysis of SsAP2-like nucleotide sequence and confirmation of miR172a-guided cleavage site in SsAP2-like mRNA

(a) Phylogenetic analysis of SsAP2-like nucleotide sequences from Solanum lycopersicum (Sl), Nicotiana tabacum (Nt), Vitis vinifera (Vv), Sesamum indicum (Si), Arabidopsis thaliana (At), Brassica napus (Bn), and Oryza sativa (Os). Phylogenetic tree was generated by the neighbor-joining method and the p-distance model using MEGA4 software. The numbers represent the bootstrap values. (b) Experimental confirmation of miR172 cleavage site in SsAP2-like mRNA. The cleavage site was determined by RLM 5'-RACE. M: marker; GSP: gene-specific primer; NTC: no-template control; NPC: no primer control. The arrow indicates the cleavage site of SsAP2-like and the number above the sequences indicates the ratio of 5'-RACE clones