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. 2018 Jul 5;39(10):1245–1253. doi: 10.1093/carcin/bgy090

Figure 4.

Figure 4.

Down regulation of EPAC1 modulates intracellular scaffolding support. (A) Representative confocal image depicts expression and localization of tubulin, MENA in siRNA-treated cells. (a) Overlay of Tubulin and MENA (control); enlarged inset image (1) Immunostained by MENA; (2) Immunostained by Tubulin (3) Merged MENA and Tubulin. (b) Overlay of Tubulin and MENA (siEPAC1); Enlarged inset image (1) Immunostained by MENA; (2) Immunostained by Tubulin (3) Merged image of MENA and Tubulin. (B) Western blot analysis was performed on siEPAC1 and siControl TNBC cells to profile expression of MENA and tubulin. (C) Representative immunofluorescence of actin and paxillin: (a) overlay of paxillin and actin (Control); Enlarged inset (1) immunostained by actin; (2) immunostained by paxillin; (3) Merged image of actin and paxillin. (b) Overlay of actin and paxillin (siEPAC1); Enlarged inset (1) Immunostained by paxillin; (2) Immunostained by actin; (3) Merged image of paxillin and actin. (D) Western blot analysis was performed on siEPAC1 and siControl TNBC cells to profile expression of MMP9, actin and paxillin.