Figure 5.

Down-regulation of EPAC1 in tumor cells prevents the increase in permeability in primary endothelial cells (ECIS). Representative trace showing HUVECs plated on 8W10E microchips, pre-coated with gelatin. After 24 h conditioned media of EPAC1, siRNA transfected cells along with negatively transfected cells were poured in the wells. HUVEC without any conditioned media were used as control for treated cells. In one well 8-OME cAMP treatment was given for 20 min and after removal of 8-OME cAMP, CM of EPAC1 transfected cells were used. Effect on changes in electric impedance of the monolayer was taken at every sec. Cell permeability analysis was observed until 48 h.