Table 3.

Putative DNA pol η mutation hotspots in lacZ

Sequence	Hotspot position	Type of changes	Number of mutations
CAATT	3	A→G,T,C	15,1,1
TTATC	14	A→G,C,T	14,1,1
GTTAT	15	T→G,A	10,5
AAATT	20	A→G,T	11,1
GAAAT	21	A→G,T	16,2
ATAGC	38	A→G,T,C	9,2,1
CATAG	39	T→G,A,C	9,9,2
TCATG	46	A→G,T	13,1
GTAAT	50	A→G,T	16,4
GAATT	56	A→G	17
AAACG	70	A→G,T	18,3
GTAAA	73	A→G,T	14,1
CAACG	77	T→C,G	12
CGACG	80	A→G,T	11,2
WA	Consensus

Note: Hotspot positions are underlined. The mutational spectrum shown in Figure 1 was converted to the complementary orientation.