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. 2017 Nov 4;39(2):98–108. doi: 10.1093/carcin/bgx126

Figure 2.

Figure 2.

MiR-513a-5p impairs the expression of PR in luminal breast cancer cell lines. (A) qRT-PCR analysis of miR-513a-5p expression levels in MCF-7 and T47D cell lines. (B, C) Western blot analysis and qRT-PCR analysis of PR expression levels in MCF7 cells transiently transfected with miR-513a-5p mimic or control. (D) Western blot analysis of PR protein expression in ZR-75-1 and BT-474 breast cancer cell lines transiently transfected with miR-513a-5p mimic or control. (E) Western blot analysis of PR protein expression in serum-starved MCF-7 cells treated for 48 h with 10 nM E2 upon miR-513a-5p over-expression. (F, G) qRT-PCR analysis of PR and ATP1B1 expression levels in serum-starved MCF-7 cells treated for 48h with 10nM E2 upon miR-513a-5p over-expression. (H) Western-blot analysis of PR protein expression in serum-starved MCF-7 cells treated for 48 h with 10 nM E2 upon miR-513a-5p inhibition (inh miR-513a-5p). (I, J) qRT-PCR analysis of PR and ATP1B1 expression levels in serum-starved MCF-7 cells treated for 48 h with 10 nM E2 upon miR-513a-5p inhibition (inh miR-513a-5p). *P < 0.05.