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. 2018 May 16;27(16):2805–2816. doi: 10.1093/hmg/ddy189

Figure 5.

Figure 5.

Inhibition of p53 prevents Nedd4-2 dephosphorylation and restores GluA1 ubiquitination in Fmr1 KO neurons. (A) Western blots (left) and quantification (right) of phospho-Akt-S473, total Akt, phospho-Nedd4-2-S342, phospho-Nedd4-2-S448, total Nedd4-2, phospho-Mdm2-S163, total Mdm2, p53 and internal control Gapdh from WT or Fmr1 KO cortical neuron cultures treated with vehicle (DMSO), PTX, pifithrin-α, or PTX + pifithrin-α for 48 h (n = 3–5 independent cultures). (B) Western blots of Ub after IP using anti-GluA1 antibody from WT or Fmr1 KO cortical neuron cultures treated with DMSO, PTX, pifithrin-α or PTX + pifithrin-α for 48 h (n = 4). Data were analyzed by two-way ANOVA with Tukey’s test and represented as mean ± SEM with *P < 0.05, **P < 0.01 and ns: non-significant.