Skip to main content
. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: Neurobiol Learn Mem. 2018 Mar 15;154:136–140. doi: 10.1016/j.nlm.2018.03.016

Fig. 1.

Fig. 1.

Sleep deprivation impairs synaptic tagging. C57BL/6J mice were deprived of sleep for 5 h by gentle handling (SD) or were left undisturbed in their home cages (NSD). In vitro field recordings were made in hippocampal slices taken immediately following the deprivation period. (A) Two stimulating electrodes were positioned along the Schaffer collateral fiber pathways in such a way as to activate two separate sets of stimulus inputs (S1 and S2) onto the same postsynaptic population of neurons in CA1. (B and C) In S1, at 0 min, strong stimulation of the massed 4-train type (four 1 s, 100 Hz trains of electrical stimuli with a 5-s inter-train interval) produced normal long-lasting potentiation in NSD and SD mice. (D and E) In S2, at 30 min, weak stimulation (one 1 s, 100 Hz train) in NSD mice resulted in long-lasting potentiation, and this synaptic tagging and capture process was impaired in SD mice. Arrows indicate timing of tetanic stimulation. Error bars indicate SEM. * indicates a post hoc test with p > 0.05.