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. 2019 Apr 8;12:159. doi: 10.1186/s13071-019-3421-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Melting peaks (a), normalized difference curves (b) and the normalized and temperature-shifted difference plot (c) of the amplified product of control species (B. malayi, B. pahangi and D immitis/D. repens) and S1-S11 representative DNAs from 34 mf-positive samples of cat blood and DNA from the 22 mf-positive samples using cox1 specific primers for D. repens, S1; S2; S5 represented the 22 mf-positive cat samples (d), as obtained with the LightCycler 480 gene scanning software. Abbreviations: Bm, B. malayi; Bp, B. pahangi; Di, D immitis/D. repens