Fig. 4.

Degradation of NETs decreases histone citrullination in endotoxemic mice. a Padi4 mRNA expression in the lung tissue from mice 18 h after LPS injection (35 mg/kg, i.p.) and treatment with or without rhDNase (10 mg/kg, s.c.). b Western blot and c representative images of immunofluorescence of histone H3 citrullination in the lungs of infant or adult mice 18 h after LPS injection. Bars = 50 μm. Data are mean ± SEM, n = 5–6 per group, ***p < 0.001 (one-way ANOVA, Bonferroni’s)