Figure 1.

UtMVECs express EC markers. (A) RNA was isolated from HUVECs and UtMVECs (Passage 5) and reverse transcribed into cDNA. RT-PCR was performed using primers specific for Claudin-5, platelet endothelial cell adhesion molecule-1 (PECAM-1), vascular endothelial-cadherin (VE-cadherin), von Willebrand factor (vWF), Vimentin, Caveolin-1, human GAPDH, cytokeratin-8 and smooth muscle actin (α-SMA). (B) Protein extracts were analyzed by Western blotting using antibodies directed to Claudin-5, PECAM-1, VE-cadherin, vWF, Vimentin, Caveolin-1, GAPDH and α-SMA.