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. 2017 Dec 21;24(2):74–93. doi: 10.1093/molehr/gax064

Figure 8.

Figure 8

Silencing MT1-MMP with shRNA in UtMVECs significantly decreases invasion responses. UtMVECs were either not transduced (non-transduced) or transduced with lentiviruses delivering shRNA directed to beta 2 microglobulin (shβ2M) or MT1-MMP (shMT1-MMP). After 4 days, UtMVECs were allowed to invade in the presence of S1P+GF (B and C) or WSS+S1P+GF (D and E). (A) Western blot analysis of MT1-MMP and β2M expression along with corresponding loading controls for each. Photographs of UtMVEC invasion responses observed in each treatment group when stimulated with (B) S1P+GF or (D) WSS+S1P+GF. Arrows indicate the original monolayer. Scale bars equal 200 μm. Invasion density (±SD) of cells was quantified manually in experiments applying (C) S1P+GF or (E) WSS+S1P+GF. Statistical significance of invasion density between Non-transduced, shβ2M and shMT1-MMP treatments was analyzed using a one-way ANOVA with Tukey’s post hoc test where each letter represents a significant statistical difference at P < 0.05 compared to other letters. Results from a representative experiment (n = 3) are shown.