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. 2018 Jan 23;24(3):143–157. doi: 10.1093/molehr/gay003

Figure 1.

Figure 1

Oviductosomes (OVS) are evolutionarily conserved in humans and carry PMCAs. (A) TEM of negatively stained human OVS showing immunogold labeling of PMCA4. (a) The IgG control shows a microvesicle (**), larger OVS (0.1–1 μm) and an exosomes (*) <100 nm. (b) Immunogold labeling (6 nm gold particles) of PMCA4 (arrows) showing gold particles on OVS. These particles are seen only on the background in (a). The inset in (b) is enlarged in (c) to show the particles (bar = 100 nm). (B, C) Western blots show the presence of HSC70, an extracellular vesicle (EV) biochemical marker, after stripping and re-probing of the membrane, as well as PMCA1 and eNOS (in dimeric and monomeric forms) in human OVS (HOVS) and human oviductal tissues (HOVD).