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Murine OVS carry in their cargo PMCA4 partners (nNOS and CASK) which interact with PMCA1. (A) WT and Pmca4−/− OVS show the 155 kDa nNOS band and ~100 kDa CASK band, with testis as positive controls. (B) PMCA1 (139 kDa) co-immunoprecipitates (co-IP) with nNOS in OLF. (C) PMCA1 co-IP with CASK in OLF. For both (B and C), Total protein (TP), obtained from OLF and rabbit IgG were used as a positive and negative control.
