Table 3.

Biofilm-formation ability and biofilm-associated genes in Staphylococcus aureus isolates obtained from pasteurized milk samples.

No.	S. aureus isolates	Biofilm production assay	Biofilm production abilitya	biofilm-producing genes					adhesion genes
				icaA	icaD	icaC	bap	eno	clfA	clfB	fnbA	fnbB	fib	cna
1	Sta376	1.4715	+++	+	+	+	-	+	+	+	+	+	+	+
2	Sta1758	0.5147	++	+	+	+	-	+	+	+	+	+	+	-
3	Sta2233-0	1.2700	+++	+	+	+	-	+	+	+	+	+	+	-
4	Sta2333-0	0.8515	++	+	+	+	-	+	+	+	+	+	+	-
5	Sta1142	1.1548	+++	+	+	+	-	+	+	+	+	+	+	+
6	Sta3008C2	0.5417	++	+	+	+	-	+	+	+	+	+	+	+
7	Sta3032	0.6850	++	+	+	+	-	+	+	+	+	+	+	+
8	Sta3032C3	1.1167	+++	+	+	-	-	+	+	+	+	+	+	-
9	Sta3532	1.0995	+++	+	+	+	-	+	+	+	+	+	+	-
10	Sta3608	2.5659	+++	+	+	+	-	+	+	+	+	+	+	-
11	Sta4032B2	2.6347	+++	+	+	+	-	+	+	+	+	+	+	+
12	Sta4032B3	2.7753	+++	+	+	+	-	+	+	+	+	+	+	+

^aQuantification of biofilm formation by optical density (OD) analysis: (+++): strong biofilm producers (OD₅₉₀ > 0.88), (++): moderate biofilm producers (0.44 > OD₅₉₀ > 0.88), (+): weak biofilm producers (0.22 > OD₅₉₀ > 0.44).