Figure 3.

Whole-transcriptome sequence analysis of Gprc5a^−/− Kras-mutant LUAD G1 spheres and parental cells. RNA-Seq was performed using the NovaSeq 6000 platform from Illumina as described in the Materials and Methods section. (A) Differentially expressed transcripts (n = 2,600) between MDA-F471 G1 spheres and parental cells (three biological replicates/independent experiments in each group) were identified using statistical criteria detailed in the Materials and Methods section and analyzed by hierarchical clustering in the R statistical language and environment. Columns represent samples and rows indicate transcripts (yellow, up-regulated relative to the median; blue, down-regulated relative to the median). (B) Representative canonical pathways differentially modulated in MDA-F471 G1 spheres relative to their parental counterparts. Modulation of canonical pathways was identified by IPA (see Methods). Select pathways are ordered from left to right based on statistical significance (indicated by –log base 10 of the P-value).