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. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: Biomaterials. 2018 Aug 14;182:279–288. doi: 10.1016/j.biomaterials.2018.08.028

Figure 1. Layer-by-layer assembly of electrospun nanofibrous TEP construct.

Figure 1.

Schematic illustration of fabrication (A), seeding (B), and assembly of the 3D nanofiber/cell construct (C). Gross appearance of the flexible tissue membrane (D). MPLSM images show stacked 3D construct with GFP+ BMSCs (E) embedded in rich SHG+ collagen matrix (F). GFP, green; SHG, white. Histologic H&E (G) and Picrosirius Red staining (H) show a multi-layered cellular construct at day 2 following assembly.