FIG 3.

MCs play a central role in NAD⁺ -mediated CD4⁺ T-cell differentiation. C57BL/6 WT and WBB6F1/JKit^w/Kit^w-v (MC-deficient) mice were treated daily with intraperitoneal injection of 40 mg of NAD⁺ or a placebo solution (PBS), as indicated. After 7 days, mice were killed, and systemic levels of CD4⁺IFN-γ⁺, CD4⁺IL-4⁺, and CD4⁺IL-17A⁺ cells were assessed by using flow cytometry. Data were derived from 2 independent experiments (n = 5–10). Data represent means ± SDs. ANOVA was used to compare groups: ***P < .001. APC, Allophycocyanin; PE, phycoerythrin.