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Unique MC gene expression profile after NAD+ activation. BMMCs from C57BL/6 mice were cultured in the presence of NAD+ (500 µmol/L), LPS (10 µg/mL; Escherichia coli O127:B8), or placebo (PBS). After 16 hours of culture, cells were collected and RNA was extracted for RNA sequencing analysis. A, Differential gene expression. B, Gene heat map expression profile. C, Ingenuity Pathway Analysis.
