Antibodies
|
| Rabbit anti-dSK (polyclonal antibody targeting SK’s N-terminal domain) |
Patrick Dolph (Abou Tayoun et al., 2011) |
N/A |
| Mouse anti-V5 |
Thermo Fisher Scientific |
Cat# R960–25;RRID:AB_2556564 |
| Rabbit anti-GFP |
Thermo Fisher Scientific |
Cat# A-11122;RRID:AB_221569 |
| Rabbit anti-HRP |
Jackson ImmunoResearch Laboratories |
Cat# 323–005-021;RRID:AB_2314648 |
| Alexa Fluor. 488 goat anti-mouse |
Thermo Fisher Scientific |
Cat# A-11029;RRID:AB_2534088 |
| Alexa Fluor. 488 goat anti-rabbit |
Thermo Fisher Scientific |
Cat# A-11034;RRID:AB_2576217 |
| Cat# A-11008;RRID:AB_143165 |
| Alexa Fluor. 546 goat anti-mouse |
Thermo Fisher Scientific |
Cat# A-11030;RRID:AB_2534089 |
| Alexa Fluor. 546 goat anti-rabbit |
Thermo Fisher Scientific |
Cat# A-11035;RRID:AB_2534093 |
|
Deposited Data
|
|
SK-V isoform sequence |
GenBank |
MH001552 |
|
Experimental Models: Organisms/Strains
|
|
D. melanogaster: RNAi strains targeting K+ channels (See Table S1) |
Vienna Drosophila Resource Center; Bloomington Drosophila Stock Center |
See Table S1
|
|
D. melanogaster: y1
w*; md-GAL4 (aka GAL4109(2)80)
|
Bloomington Drosophila Stock Center |
BDSC: 8769; FlyBase: FBti0015554 |
|
D. melanogaster: y1
w*; md-GAL4 (aka GAL4109(2)80)
|
Bloomington Drosophila Stock Center |
BDSC: 8769; FlyBase: FBti0015554 |
|
D. melanogaster: w*; ppk-GAL4 (aka ppk1.9-GAL4)
|
Ainsley et al., 2003 |
FlyBase: FBal0152201 |
|
D. melanogaster: w1118; UAS-Dicer2
|
Vienna Drosophila Resource Center |
60009 |
|
D. melanogaster: w;; UAS-GCamP3.0attp2
|
Bloomington Drosophila Stock Center |
BDSC: 32236 |
|
D. melanogaster: y1
w67c23;; attp2
|
Bloomington Drosophila Stock Center |
BDSC: 8622; FlyBase: FBti0040535 |
|
D. melanogaster: w*
P{XP}d01963
|
The Exelixis Collection at the Harvard Medical School |
d01963; FlyBase: FBti0054688 |
|
D. melanogaster: w*
pBac{WH}SKf01403
|
The Exelixis Collection at the Harvard Medical School |
f01403; FlyBase: FBst1016953 |
|
D. melanogaster: w1118
ΔSK
|
Generated in this study |
N/A |
|
D. melanogaster: w1118
SK::V5
|
Generated in this study |
N/A |
|
D. melanogaster: w1118 ;
UAS-SK-M
|
Generated in this study |
N/A |
|
D. melanogaster: w1118 ;
UAS-SK-V
|
Generated in this study |
N/A |
|
Oligonucleotides
|
| See Table S3
|
N/A |
N/A |
|
Recombinant DNA
|
| BAC: CH321–90C05 (aka CHORI-321–90C05) |
BACPAC Resources Center (BPRC) |
CH321–90C05 |
| TOPO-XL |
Thermo Fisher Scientific |
Cat# K8050 |
|
pENTR (Gateway Entry Vector) |
Thermo Fisher Scientific |
Cat# K240020 |
|
pTW (Drosophila Gateway Vector Collection) |
Drosophila Genomics Resource Center |
Gateway Collection |
