Figure 8.

Nanoparticle uptake assay in HeLa and HEK-293 cells. To demonstrate that nanoparticles can be internalized into normal or cancer cells, fluorescent ANPs+ATP labeled with Alexa Fluor 488 (A) and EM-ANPs+ATP labeled with DiD (B) were prepared. Analysis by confocal microscopy revealed a lower number of internalized EM-ANPs+ATP nanoparticles in normal and cancer cells (C), suggesting a longer circulation time for these nanoparticles. A higher proportion of ANPs+ATP and EM-ANPs+ATP was observed in HeLa cells vs HEK-293 cells Data are shown as mean ± SEM (n=50), *P-value ≤0.05, ***P-value ≤0.001.

Abbreviations: ANPs+ATP, ATP-carrying albumin nanoparticles; EM-ANPs+ATP, ATP-carrying albumin nanoparticles coated with erythrocyte membranes; SEM, standard error of mean.