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Schematic chart of the design of the different primer pairs for the paired quantitative polymerase chain reaction assays. For MIE gene, LF1/LR1 = 1640 bp, LF2/LR1 = 578 bp, SF1/SR1 = 138 bp, SF1/SR2 = 81 bp; probe was located in between SF1 and SR2. For gB gene, F265/R2658 = 2394 bp, F2074/R2658 = 585 bp, F2074/R2143 = 70 bp.
