Figure 4.

Results of cytomegalovirus (CMV) DNA quantitation of 20 clinical plasma samples collected from 12 solid organ transplant recipients using 3 paired quantitative polymerase chain reaction assays with long and short amplicons. Each unique patient is identified by a number; serial samples collected from the same patient are identified as a, b, and c. The mean, median, and (range) of CMV viral load expressed as log₁₀ copies per milliliter for the 20 samples were as follows: Taqman MIE 138 bp, 5.46, 5.52 (4.60–6.26) versus Taqman MIE 81 bp, 5.87, 5.89 (5.34–6.37) (A); SYBR MIE 578 bp, 5.20, 5.16 (4.82–5.84) versus SYBR MIE 138 bp, 5.74, 5.76 (5.18–6.51) (B); SYBR gB 585 bp, 5.41, 5.42 (4.82–6.55) versus SYBR gB 70 bp 6.23, 6.26 (5.44–6.92) (C). The mean log₁₀ copies per milliliter difference between the short and long amplicon assays in A, B, and C were 0.41 (P < .001), 0.54 (P < .001), and 0.81 (P < .001), respectively. Abbreviation: CMV, cytomegalovirus.