Table 2.
Results of Testing a Fresh Plasma Sample and a Frozen Plasma Sample From 2 Solid-Organ Transplant Recipients Compared With a Control Sample Using the 3 Paired Quantitative Polymerase Chain Reaction Assays
| Sample type | Patient/ sample | Sample storage | P-qPCR-1 (Taqman MIE), log10 copies/mL | P-qPCR-2 (SYBR MIE), log10 copies/mL | P-qPCR-3 (SYBR gB), log10 copies/mL | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 81 bp |
138 bp |
Log10 differencea | 138 bp |
578 bp |
Log10 difference | 70 bp |
585 bp |
Log10 difference | |||
| Fresh plasma | D+/R− 11 months after kidney transplant/no antiviral therapy |
4oC 48 h |
6.78 | 5.53 | 1.25 | 6.33 | 4.79 | 1.54 | 6.92 | 5.44 | 1.48 |
| Frozen plasma | D+/R− 3 months after multivisceral transplant/no antiviral therapy |
−70oC 3 months |
6.56 | 6.36 | 0.20 | 6.17 | 5.95 | 0.22 | 6.71 | 5.79 | 0.92 |
| Frozen control | CMV negative plasma spiked with Merlin virus stock | −70oC 6 months |
5.43 | 5.37 | 0.06 | 5.21 | 5.27 | –0.06 | 5.57 | 5.41 | 0.16 |
Abbreviations: CMV, cytomegalovirus; D, donor; P-qPCR, paired quantitative polymerase chain reaction; R, recipient.
alog10 difference = smaller amplicon qPCR measurement result - larger amplicon qPCR measurement result.