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. 2016 Dec 8;215(3):456–465. doi: 10.1093/infdis/jiw569

Figure 4.

Figure 4.

A, Bar chart of lipid content of C. muridarum MoPn infected and noninfected C57epi cells. The infection of C57epi cells with MoPn (MOI 5) resulted in phospholipid upregulation as measured by the increase in choline content of infected and noninfected cells. B, Western blot analysis of ATF4 activation during Chlamydia infection. Ten μg total protein from C57epi cells infected with C. muridarum (MoPn) for 24, 48, and 72 hours were prepared for Western blot analysis. Blot was probed with primary antibody against ATF4. Lane 1: protein molecular weight marker; Lanes 2–4: samples from noninfected C57epi cells at 24, 48, and 72 hours. Lanes 5–7: samples from C57epi cells infected with C. muridarum (MOI 1) at 24, 48, and 72 hours. Lanes 8–10: samples from C57epi cells infected with C. muridarum (MOI 5) at 24, 48, and 72 hours. Abbreviations: ATF4, activating transcription factor 4; C57epi cells, mouse oviduct epithelial cells; MOI, multiplicity of infection; MoPn, mouse pneumonitis; NI, noninfected.