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. 2019 Mar 29;10:573. doi: 10.3389/fmicb.2019.00573

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Copyright © 2019 Lombardi, Tolchard, Bouillot, Signor, Gebus, Liebl, Fenel, Teulon, Brock, Habenstein, Pellequer, Faudry, Loquet, Attrée, Dessen and Job.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence alignment of needle proteins from different bacteria. PscF from Pseudomonas aeruginosa, YscF from Yersinia sp., MxiH from Shigella sp., PrgI from Salmonella sp. The residues mutated in this study are indicated with a green asterisk. Numbers refer to the PscF sequence. Secondary structure elements from the PrgI structure are indicated underneath the sequence alignment. The figure was generated with ESPript, using the new ENDscript server (Robert and Gouet, 2014). Conserved and similar residues are shown in red and blue boxes, respectively.