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. 2019 Mar 29;10:573. doi: 10.3389/fmicb.2019.00573

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Copyright © 2019 Lombardi, Tolchard, Bouillot, Signor, Gebus, Liebl, Fenel, Teulon, Brock, Habenstein, Pellequer, Faudry, Loquet, Attrée, Dessen and Job.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of PscF-needles on the surface of Pseudomonas aeruginosa. (A) P. aeruginosa CHA strain isolated on cystic fibrosis patient depleted for the pscF gene (ΔpscF) and complemented with pIApG-pscF constructs were grown in T3SS-inducing conditions. PscF needles were visualized by immuno fluorescence on fixed bacteria using anti-PscF (in green) or anti-PcrV (in cyan). SYTO24 was used to visualize bacteria (in red). As a negative controls we used P. aeruginosa ΔF and ΔV, while ΔF/Fwt and ΔV/Vwt were used as a positive control. Only few PscF and PcrV spots were visible on P. aeruginosa strains carrying a PscF-D76A or a PscF-P47A/Q54A. (B) MicrobeJ reconstitution of PscF distribution around the bacteria. n = number of spots in the image.