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. 2019 Mar 29;10:530. doi: 10.3389/fmicb.2019.00530

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Copyright © 2019 Lan, Wu, Fan, Sun, Yang, Zhang, Yang, Lin, Chen, Tian and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of the set3 deleted (Δset3-1 and Δset3-2), complemented strains (Δset3-com), and growth analysis. (A) Construction strategy for Δset3 strain using homolog recombination. (B) The deleted and complemented strains were verified by PCR analysis with genomic DNA as template, lane 1~4: PCR examination on upstream of WT, Δset3-1, Δset3-2, and Δset3-com strains, lane 5~8: PCR examination on downstream of WT, Δset3-1, Δset3-2, and Δset3-com strains. (C) RT-PCR was used to confirm the transcript levels of set3 gene in deleted and complemented strains. (D) Southern blot was conducted to confirm the deletion mutants. (E) Colony morphology of WT, Δset3, and Δset3-com strains, grown on PDA media at 37°C for 5 days. (F) The Δset3-1 and Δset3-2 strain showed fluffier phenotype when compared to WT and Δset3-com strains.