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. 2019 Mar 29;10:285. doi: 10.3389/fphys.2019.00285

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Copyright © 2019 Wang, Yang, Li and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of cold temperature or/and CSE on TRPM8 expression in 16HBE cells relative quantification of transient receptor potential melastatin 8 (TRPM8) mRNA and protein in 16HBE cells exposed to18°C or/and CSE. (A) The levels of the TRPM8 protein were determined by western blot analysis. (B) The levels of TRPM8 mRNA were determined by real-time RT-PCR, and a comparative Ct method (2^−ΔΔCt) was used for the relative mRNA quantification. (C) Densitometry quantification of the bands in A was performed using Quantity One software, and the results are expressed as the ratio of the expression of TRPM8 to β-actin. The values in B and C are shown as the means ± SD; n = 4. ^∗p < 0.05 vs. control.