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. 2019 Jan 16;8(1):45–54. doi: 10.1080/22221751.2018.1558962

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — M2e-specific humoral and cellular immune responses. M2e-specific IgG, IgG1, and IgG2a titers were determined by ELISA. Cellular immune response induced by M2e-immunized mice detected by ELISpot. Data are expressed as spot-forming cells (SFCs) responding to peptide-specific IFN-γ secretion and are presented as the means ± standard deviation. [A, B, C (once immunization), D, E, F (twice immunization) and G, H, I (three times immunization)]. Statistical significance was defined as ***p < 0.001, **p < 0.01.