Figure 4.

hMxA-tg mice resist lethal dose of PR8-H18N11. (a) hMxA-tg mice were pretreated with IFN-α or PBS 18 h prior to infection with 7xLD₅₀ of PR8-H18N11 (values refer to hMxA-tg mice). Survival and changes in body weight were monitored for 14 days. Mice were sacrificed and scored dead when 75% of the initial body weight was reached. Error bars indicate the standard error of the mean. (b) Groups of B6 and hMxA-tg mice were pretreated with IFN-α or PBS 18 h prior to infection with 7xLD₅₀ of PR8-H18N11 (values refer to hMxA-tg mice). Viral organ titres of the upper respiratory tract (URT), trachea and lung were determined at the indicated time points. (c) MxA and NP protein levels in homogenized lungs from infected mice from (b) were detected by Western blot.