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. 2019 Feb 27;8(1):327–338. doi: 10.1080/22221751.2019.1581034

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — NA activities of NA-VLPs co-expressed with different levels of HA.H1N1-VLPs with different HA levels were collected from 293 T cells transfected with various DNA plasmids ratio and were normalized by the activities in NA-Star assays. (A) FLAG-tagged HA and NA protein levels (80 and 75 kDa) of the normalized VLPs were determined by western blotting using anti-FLAG monoclonal antibody. (B) Desialylation of fetuin by the VLPs was determined in ELLA. Mean absorbance at 492 nm is plotted against sample.