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Desialylation of fetuin and 3′SLN by influenza virus and NA-VLP. (A) NA activities of NA-VLP and low pH pre-treated H1N1pdm viruses were measured in ELLA. Desialylation of fetuin and 3′SLN were detected using HRP-conjugated ECA lectin. (B) Cleavage of 3′SLN in solution was evaluated in NMR spectroscopy. 1H NMR spectra were recorded at 10-minute intervals. The yield of cleavage was quantified by the ratio of different chemical shift from the protons associated with NHAc group
