Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 8;8(1):585–602. doi: 10.1080/22221751.2019.1598291

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — ZIKV NS2A mutagenesis. (A) Sequence alignment of NS2A proteins among ZIKV (strain FSS13025), DENV-1-4 serotypes, WNV, JEV, SLEV, and YFV using CLC Main Workbench software (CLC Bio). Thirty-five amino acids were selected for mutagenesis: (i) All 8 identical non-alanine residues (indicated by triangles), (ii) 25 charged residues (arrows), (iii) a K25/K26/R27 motif (line), (iv) residue N130 (circle). The consensus and conservation percentage are indicated below each amino acids. (B) A summary of ZIKV NS2A mutagenesis results. An infectious cDNA clone and a luciferase replicon of ZIKV strain FSS13025 were used to analyze the function of each amino acid. Based on the replication phenotypes, the 35 mutants could be categorized into five classes as indicated.