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. 2019 Apr 2;10:685. doi: 10.3389/fimmu.2019.00685

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Copyright © 2019 Hazeldine, Dinsdale, Harrison and Lord.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of traumatic injury on neutrophil glucose uptake and metabolism. Glucose uptake by basal (A) or PMA-stimulated (B) neutrophils isolated from healthy controls (HC) and trauma patients. MFI, Mean fluorescence intensity. (C,D) Comparison of lactate concentration in cell-free supernatants collected from resting (C) or PMA stimulated (D) neutrophils isolated from HC and trauma patients following a 3 h in vitro culture. Number of samples analyzed are indicated below each time-point. ^*p < 0.01, ^**p < 0.001, ^***p < 0.0001 vs. HC. (E,F) Expression of the glycolytic enzymes pyruvate kinase (PKM2) and lactate dehydrogenase A (LDHA) in resting neutrophils isolated from HC (n = 5) and trauma patients 48–72 h post-injury (n = 14). Data are presented as representative Western blots (E) and densitometry analysis of collated data for LDHA (F, top panel) and PKM2 (F, bottom panel). ^*p < 0.01 vs. HC.